Oops, yes, I am mixing up neutral red - and the recipe from Bryan is what I use - it last forever.
I have had luck with nuclear fast red from vector - comes made up. That lasts a little longer than lab made. mills ᐧ On Wed, Aug 8, 2018 at 12:52 PM Caroline Miller <mi...@3scan.com> wrote: > HI Tasha, > > The nuclear fast red will stain the nuclei red, the biebrich scarlet is a > connective tissue stain and may cover up the (sometimes sparse) prussian > blue localization of iron in the tissues. I, personally, would not consider > these reagents swappable. > > In my experience nuclear fast red does last a long time, but I am also not > in a lab where expiration dates matter. > > mills > ᐧ > > On Wed, Aug 8, 2018 at 4:52 AM Campbell, Tasha M. via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > >> Is nuclear fast red the only counter stain for Prussian blue stain? I >> have a Masson's trichrome kit and was wondering if the Bierbech Scarlet >> could be a counterstain. I won't be doing the iron stain very often at all >> so I am trying to keep dry reagents on hand to make up as needed so they do >> not expire so quickly. Thanks! >> >> >> >> >> Tasha Campbell, B.S.,HTL(ASCP) >> Frederick Gastroenterology Associates >> 310 W. 9th St. >> Frederick, MD 21701 >> 301-695-6800 ext. 144 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > -- > Caroline Miller (mills) > Director of Biology > > www.3Scan.com > 415 2187297 > > > -- Caroline Miller (mills) Director of Biology www.3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet