I will try the safranin O on two samples. Also, toluidine blue has been suggested. Do you have any experience with it?
Betsy Molinari Sr. Histology Research Technician CV Pathology Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: bmolin...@texasheart.org texasheart.org | facebook | twitter -----Original Message----- From: Lynette Pavelich [mailto:lynette...@gmail.com] Sent: Friday, April 26, 2019 9:20 AM To: Betsy Molinari <bmolin...@texasheart.org> Subject: Re: [Histonet] biopsy staining *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ I can’t think of a reason why it wouldn’t work or hurt. Give it a try! Dab off the alcohol and mark. Let us know how it worked out in the end! > On Apr 26, 2019, at 10:04 AM, Betsy Molinari <bmolin...@texasheart.org> wrote: > > That was my concern as well. Since the tissue is already in 70% ETOH can it > still be marked? > > > Betsy Molinari > Sr. Histology Research Technician > CV Pathology > > Texas Heart Institute > 6770 Bertner Avenue, MC 1-283 > Houston, TX 77030 > > Office: 832-355-6524 | Fax: 832-355-6812 > Email: bmolin...@texasheart.org > texasheart.org | facebook | twitter > -----Original Message----- > From: Lynette Pavelich [mailto:lynette...@gmail.com] > Sent: Friday, April 26, 2019 7:55 AM > To: Betsy Molinari <bmolin...@texasheart.org> > Subject: Re: [Histonet] biopsy staining > > *** Important*** This email is not from Texas Heart Institute. Only click > links or open attachments you know are safe. > ________________________________ > > > I would not choose eosin then because of its fluorescent properties. > > Lynette Pavelich, HT(ASCP), QIHC > > Sent from my iPhone > >> On Apr 26, 2019, at 8:21 AM, Betsy Molinari via Histonet >> <histonet@lists.utsouthwestern.edu> wrote: >> >> Hi, >> I have researched the archives but still have concerns. I have some very >> small pieces of tissue that are in 70% ETOH. Should I mark them with eosin >> or safranin O? From what I have read the tissues were still in formalin, and >> mine are already in alcohol. Can they still be marked? At the moment they >> are not planning to do florescence, but I would like to keep that option >> open. >> Thanks, >> Betsy Molinari, HT (ASCP) >> Texas Heart Institute >> 6770 Bertner Ave >> Houston, TX 77030 >> 832-355-6524 (lab) >> 832-355-6812 (fax) >> >> Betsy Molinari >> Sr. Histology Research Technician >> CV Pathology >> >> Texas Heart Institute >> 6770 Bertner Avenue, MC 1-283 >> Houston, TX 77030 >> >> Office: 832-355-6524 | Fax: 832-355-6812 >> Email: bmolin...@texasheart.org >> texasheart.org<https://www.texasheart.org/> | >> facebook<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.facebook.com_Texas.Heart.Institute&d=DwIFAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=cHhb45byVhOxSFbivksM2zWEfl4MWs5JTZK6OYKxZlU&s=gKyFrcWIRiP3OTwKv2i6IcMuxfq8Afj820MTKCAaDZg&e=> >> | >> twitter<https://urldefense.proofpoint.com/v2/url?u=https-3A__twitter.com_Texas-5FHeart&d=DwIFAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=cHhb45byVhOxSFbivksM2zWEfl4MWs5JTZK6OYKxZlU&s=M4glgc32FbpvGW6sdITwbAAhH-1fgK9JgCxPvnMYFyE&e=> >> >> This email and attachments contain information that may be confidential or >> privileged. If you are not the intended recipient, notify the sender at once >> and delete this message completely from your information system. Further >> use, disclosure, or copying of information contained in this email is not >> authorized, and any such action should not be construed as a waiver of >> privilege or other confidentiality protections. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIFAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=cHhb45byVhOxSFbivksM2zWEfl4MWs5JTZK6OYKxZlU&s=K9RNgsjqcWmyx9OrsTyV8PuXkc0lvho75ejDhZMUfAo&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
