Hi Are you processing to Pwax? If so, I stain my mouse DRGS ( very small) using Haemalum. 5 mins max ( I use Gill's I) then blue in alkaline tapwater for 10 mins before Pwax processing Stain from Formalin or alcohol but.....rinse in dist water to remove before staining. The "blue" stays nicely in the tissues, for embedding : sure, I then subject the sections to HIER without pretreatment as the citric acid treatment gets rid of any dye. If doing dye-staining I will acid-alcohol treat the sections before staining. Works well for me.
Good luck Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
