In my experience; If the the specimen is under-fixed or under-processed, the section will be sloughed off during IHC staining.
I find proper fixation time and drying the slides at room temperature (before baking them in oven) optimal. Andy Abdulbasit Andijany (Andy) AMLS, BBMS, MSc, CT (ASCP)CM, HTL (ASCPI) King Fahad Armed Forces Hospital, Jeddah, Saudi Arabia On 26 Feb 2020, at 18:35, John Garratt via Histonet <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> wrote: Do you float and pick up your sections from a water bath of distilled water with no adhesive added? If not, doing that will help. John On Wed, Feb 26, 2020 at 6:41 AM, Charles Riley via Histonet <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> wrote: Our pathologists are complaining that chunks of the dermis are missing from IHC slides yet the entire section is present prior to staining. Does anyone have any ideas what could cause the tissue to not adhere to the slides throughout the staining process? We use the Leica Bond stainers. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
