Are you using some form of antigen retrieval? In addition to what has been discussed previously, antigen retrieval using heat (HIER) can cause tissue loss especially collagen.
Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) richard.car...@hhchealth.org -----Original Message----- From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, February 26, 2020 9:44 AM To: Histo List Subject: [Histonet] IHC troubleshooting EXTERNAL email from Outside HHC! Do NOT open attachments or click links from unknown senders. Our pathologists are complaining that chunks of the dermis are missing from IHC slides yet the entire section is present prior to staining. Does anyone have any ideas what could cause the tissue to not adhere to the slides throughout the staining process? We use the Leica Bond stainers. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=DSmBsjGihQbMxzBLaqCbr8whSAZVh5SjOAr5sVbspE8&s=Bs1CxoLt5gMxm60Er8yfxoTB45uLgwau1GA_3MK5-jQ&e= Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
