Make sure they sit at room temperature longer than normal and then dry them longer. I found that cutting them thinner (3 microns) also helps.
Ginny Kurth, HT, (ASCP)CM UW Health Surgical Pathology 1111 Highland Ave Madison, WI -----Original Message----- From: Hobbs, Carl via Histonet <histonet@lists.utsouthwestern.edu> Sent: Tuesday, March 01, 2022 1:30 PM To: histonet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] IHC staining of cartilage WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Yes...a big problem for me, too....if you are using HIER?? Cartilage and bone are major tissue retention problems I cut Pwax sections of mouse long bones/knee joints for anti GFP IHC I still get a % fall off but, using manual HIER at 90C I manage to get enough sections to get good results However, I imagine that your autoIHC stainer prob uses 90C HIER? I find Trajan 3 series slides more reliable than Superfrost Plus slides. Sure, I need to dry my slides for 24 hrs in an operating fume hood ( we can't turn ours off/down) Then I place slides in a 60C oven for 2hrs. Then dewax etc.... Be interesting to read of other suggestions Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
