We use P8920 Sigma-Aldrich Poly-L-lysine solution. Great retention without excess background staining. You can prep a rack of slides in diluted solution but we put a gloved finger over the end of the bottle and and swipe it down the slide at full strength. Let the slide dry and move forward as you normally would.
Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786 Fax: 413-322-4790 charles.ba...@baystatehealth.org -----Original Message----- From: histonet-requ...@lists.utsouthwestern.edu <histonet-requ...@lists.utsouthwestern.edu> Sent: Wednesday, March 2, 2022 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 220, Issue 2 ⚠ EXTERNAL EMAIL Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!DNKm7xijBw!QHRtbD7GLZW7UOIDd0PZve5c_AlDixDOj3pnc7epMKm8jxv4F9_wxmfqmGVNeJGbQ9lUKw$ or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." EXTERNAL EMAIL: This email came from outside of Baystate. Please use extreme caution before clicking on any links or opening any attachments. ---------------------------------------------------------------------- Please view our annual report at http://www.bhannualreport.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at 413-794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet site at https://www.baystatehealth.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet