Hi Mikk - this is the right place :)
On 02/12/2014 17:40, Mikk Puustusmaa wrote:
Problem is that I would like to link protein multiple sequence
alignment (MSA) with its corresponding DNA alignment, meaning that, if
I click on the amino acid in some sequence in protein MSA view then
the corresponding codon (three nucleotides) should be highlighted by
jalview on the follower view.
interesting ! I can tell you right now that there are no applet
examples currently available that do this, but we're very keen to create
some :)
Simpler solution would be for example, if I click on the second amino
acid in some protein sequence in MSA then the fourth nucleotide should
be highlighted (maybe n*3-2 correction?). At the moment if I enter a
protein MSA to main view and corresponding nucleotide MSA to follower
view and I select a protein at second position then second nucleotide
highlights on follower view.
What you suggest is exactly what's needed. The 'two jalviewLites'
example simply echos events from one applet to the other, without any
processing. If your DNA alignment only consist of codons then it is sort
of straightforward to perform mouseover highlighting, since
JalviewLite's mouse over message gives you both sequence and alignment
column position, and you can specify whether you want to highlight a
position in sequence or alignment reference space:
jvfollower.highlight("FER1_MESCR","70","false") // residue 70
jvfollower.highlight("FER1_MESCR","70","true") // column 70
The only thing missing is to highlight a whole codon for a particular
sequence. That's not too hard to implement, though.
Selection is more tricky. JalviewLite works with columns for specifying
regions to select, so you would need to do the column position
correction yourself - and account for misaligned codons if necessary.
There is another way to do all this. Jalview includes mechanisms for
mouseover highlighting of translated DNA->protein alignments, so that
could also be reused for your example. The applet would need to be
customised for the purpose, since you'd need to specify your coding
region alignment, the products, and how they map, but the display could
then be done in a single Jalview applet, avoiding the need to create
javascript communication hooks.
let me know what you'd prefer to do !
Jim.
_______________________________________________
Jalview-discuss mailing list
[email protected]
http://www.compbio.dundee.ac.uk/mailman/listinfo/jalview-discuss
