GIMP + GAP (GIMP Animation Package) can provide you the needed tools.
You can even configure GIMP dialogs and toolbar to look pretty much like Pixen
GIMP has a good set of tools for pixel art and work with indexed palettes.

To the rest: Although it is true that Ubuntu isn't 100%, I don't think
this thread is the place to discuss such thing. It sounds more
friendly to suggest a 100% libre distro rather than bashing people
because they don't use one.
In that case, I'd say that Trisquel is a good recommendation, since,
being an Ubuntu derivative, it provides the majority of the "user
friendly" features with 100% free packages.
For a user who doesn't need 3D acceleration and can live with a
partial Flash support, it's probably the best 100% free gnu/linux
distro out there for newcomers.

2011/9/6 C. A. MacFinn <c...@cgmuse.com>:
> I am working for a graphics company that needs an application that will make 
> sprite sheets and animated gifs.  We have been using Pixen on the Mac but are 
> switching many of our workstations to Linux.  Is there anything equivalent to 
> Pixen that runs well on Ubuntu?
>
> Thanks,
>
> C. A. MacFinn
>

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