Dear MARMAM members,
On behalf of all the authors, I would like to share with you our last paper published in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology: *Transcriptome analysis of cadmium exposure in kidney fibroblast cells of the North Atlantic Right Whale (Eubalaena glacialis) *by Ierardi JL, Velosa A, Mancia A. *Abstract:* An 8X15k oligonucleotide microarray was developed consisting of 2334 Eubalaena glacialis probes and 2166 Tursiops truncatus probes and used to measure the effects, at transcriptomic level, of cadmium exposure in right whale kidney fibroblast cells. Cells were exposed to three concentrations (1 μM, 0.1 μM, and 0.01 μM) of cadmium chloride (CdCl2) for three exposure times (1, 4, and 24 h). Cells exposed to 1 μM CdCl2 for 4 h and 24 h showed upregulated genes involved in protection from metal toxicity and oxidative stress, protein renaturation, apoptosis inhibition, as well as several regulators of cellular processes. Downregulated genes represented a suite of functions including cell proliferation, transcription regulation, actin polymerization, and stress fiber synthesis. The collection of differentially expressed genes in this study support proposed mechanisms of cadmium-induced apoptosis such as ubiquitin proteasome system disruption, Ca2+ homeostasis interference, mitochondrial membrane potential collapse, reactive oxygen species (ROS) production, and cell cycle arrest. The results also have confirmed the right whale microarray as a reproducible tool in measuring differentiated gene expression that could be a valuable asset for transcriptome analysis of other baleen whales and potential health assessment protocols. The full paper is available under the following link: https://authors.elsevier.com/a/1cB-v5SMCg%7EbA1 <https://authors.elsevier.com/a/1cB-v5SMCg~bA1> Best regards, Annalaura Mancia -- *Annalaura Mancia, PhD* *Assistant Professor* *University of Ferrara* *Department of Life Sciences and Biotechnology* *Via L. Borsari, 46* *44121, Ferrara, FE, Italy* *work: +390532455704* *cell: +393421470120* *fax: +390532455715* *Email: annalaura.man...@unife.it <annalaura.man...@unife.it>* *Skype: annalaura5*
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