Hi Lunwen, It seems that your user account does not have write access to the directory where the sequences are located. Try moving them into "My Documents" or an equivalent directory in your user account. Also, is there a reason you are not using progressiveMauve for alignment? mauveAligner is only included for historical reasons, if the problem persists with progressiveMauve after changing your working directory, please write back. Best, -Aaron
On Wed, 2014-05-21 at 19:53 +1000, Lunwen Qian wrote: > Dear All, > > I was trying to compare homologous A2 (19,373,520-22,605,415 bp) with > C2 (41,712,768-44,366,288 bp) region using Mauve software. I met one > problem and did not know how to solve it. Would you give me some advices. > > Here is the log: > OS name is: Windows 7 arch: amd64 > Executing: > win64\mauveAligner --output=<aa> --island-size=50 > --island-output=<aa>.islands --backbone-size=50 --max-backbone-gap=50 > --backbone-output=<aa>.backbone --id-matrix=<aa>.id_matrix > --output-alignment=<aa>.alignment --output-guide-tree=<aa>.guide_tree > C:\Program Files\Java\A2_C2 homologous > region\chrA02_19373520_22605415.fasta C:\Program Files\Java\A2_C2 > homologous region\chrA02_19373520_22605415.fasta.sslist C:\Program > Files\Java\A2_C2 homologous region\chrC02_41712768_44366288.fasta > C:\Program Files\Java\A2_C2 homologous > region\chrC02_41712768_44366288.fasta.sslist > Sequence file appears corrupt, proceeding with caution > Sequence loaded successfully. > C:\Program Files\Java\A2_C2 homologous > region\chrA02_19373520_22605415.fasta 3231896 base pairs. > Error creating sorted mer lis > tSequence loaded successfully. > C:\Program Files\Java\A2_C2 homologous > region\chrC02_41712768_44366288.fasta 2653521 base pairs. > Using weight 15 mers for initial seeds > Sorted mer list loaded successfully > Creating sorted mer list > Exited with error code: -10 > Unhandled gnException: Exception FileNotOpened thrown from > Unknown() in ..\libMems\FileSML.cpp 125 > Unable to open file for writing. > > Best Regards > Lunwen Qian > > ------------------------------------------------------------------------------ > "Accelerate Dev Cycles with Automated Cross-Browser Testing - For FREE > Instantly run your Selenium tests across 300+ browser/OS combos. > Get unparalleled scalability from the best Selenium testing platform available > Simple to use. Nothing to install. Get started now for free." > http://p.sf.net/sfu/SauceLabs > _______________________________________________ > Mauve-users mailing list > Mauve-users@lists.sourceforge.net > https://lists.sourceforge.net/lists/listinfo/mauve-users -- Aaron E. Darling, Ph.D. Associate Professor, ithree institute University of Technology Sydney Australia http://darlinglab.org twitter: @koadman UTS CRICOS Provider Code: 00099F DISCLAIMER: This email message and any accompanying attachments may contain confidential information. If you are not the intended recipient, do not read, use, disseminate, distribute or copy this message or attachments. If you have received this message in error, please notify the sender immediately and delete this message. Any views expressed in this message are those of the individual sender, except where the sender expressly, and with authority, states them to be the views of the University of Technology Sydney. Before opening any attachments, please check them for viruses and defects. Think. Green. Do. Please consider the environment before printing this email. ------------------------------------------------------------------------------ The best possible search technologies are now affordable for all companies. Download your FREE open source Enterprise Search Engine today! Our experts will assist you in its installation for $59/mo, no commitment. Test it for FREE on our Cloud platform anytime! http://pubads.g.doubleclick.net/gampad/clk?id=145328191&iu=/4140/ostg.clktrk _______________________________________________ Mauve-users mailing list Mauve-users@lists.sourceforge.net https://lists.sourceforge.net/lists/listinfo/mauve-users
