Hi Ray,
As the error states, xmfa2maf does not support alignments that span
multiple contigs or chromosomes. This would be a nice feature to have
but isn't exactly trivial to implement. One workaround might be to
concatenate all of your sequences prior to alignment, although that may
complicate whatever you plan to do next with the MAF.

Best,
-Aaron

On Fri, 2014-05-16 at 17:53 +1000, Raymond Wan wrote:
> Dear all,
> 
> I am getting a strange error when I use xmfa2maf (downloaded from the 
> "All other programs" link).  My original FASTA file has a set of 
> sequences.  After they are all loaded (i.e., various "Sequence loaded 
> successfully." messages), I get this error:
> 
> interval 20 seq 1 left 1033547 right 1038442
> l_baseI 1033547 r_baseI 128 l_contigI 0 r_contigI 1 name ...
> Error, input alignment must spans multiple contigs/chromosomes. Unable 
> to translate to MAF
> 
> Has anyone encountered this error before?  Thank you in advance!
> 
> Ray
> 
> PS:  I had asked an unrelated question back on May 2nd about the format 
> of the SNP file.  If someone could help me out with that, that would be 
> great!  I'm actually still unsure of the file format...
> 
> 
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-- 
Aaron E. Darling, Ph.D.
Associate Professor, ithree institute
University of Technology Sydney
Australia

http://darlinglab.org
twitter: @koadman



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