The following message is official and has been approved by the
appropriate authorities to be posted to this list.
+++++++++++++
Posting for a lab Technician - Brown University
Fairbrother lab, Rm 404
Box G-E404
Brown University,
70 Ship Street
Providence RI 02912-G
Telephone: (401)863-6215
[EMAIL PROTECTED]
RESEARCH:
My lab studies the molecular recognition events necessary for
pre-mRNA splicing and also tries to understand what goes wrong with
these events in human disease.
There is a computer component and a lab component. The lab component
will be using a reporter system to identify sequences that can
function as splicing enhancers. We will pick these sequences such
that we are testing human genetic disease alleles or other sequences
that contain natural variations that we suspect will alter their
enhancer activity. Now the trick is we will want to make 4,000 of
these constructs at once and then follow them through hybridization
with microarrays. So I am in the process of getting these oligos
synthesized commercially and the libraries should be ready to go by
this summer.
The other questions will be: What are the motifs that can enhance
splicing, do these motifs work together in some situations? We will
also be identifying trans acting factors for particular sequences.
A lot of the actual day to day will be working as a team to develop
this high throughput technology. There are opportunities to do
molecular biology. There are even opportunities to do programming, if
you wish.
JOB REQUIREMENTS:
I am looking for an individual with quality molecular biology
research experience coming from any physical or life science
background. Salary will be commensurate with experience but will
definitely start higher than the institutional minimum (27K).
I do not except a fully trained applicant but an ideal candidate will
have performed research outside of the classroom. Another plus would
be proficiency in some of these techniques: PCR, hybridizations,
electrophoresis (native and denaturing PAGE, agarose , Westerns,
Northerns, Southerns), DNA preps, RNA extraction, transfection,
cloning of constructs and tissue culture.
PROVIDENCE, R.I.
Providence, the second largest city in New England, is a newly
revitalized urban center with an ethnically diverse population of
180,000. The "Renaissance City" is home to a thriving arts community,
award-winning theaters, excellent restaurants, and a rich diversity
of neighborhoods; Brown/RISD Campus is full of independent cinema
houses, coffee shops and second hand bookstores. Federal Hill, not
far from the Brown campus, is a Italian-American community offering a
variety of ethnic shops and restaurants. Large bonfires that burning
in metal urns in the Providence river while live music plays in a
carnival like atmosphere has made Waterfire the signature event
during the summer.
Dominated by Narragansett Bay, Rhode Island boasts many miles
of scenic coastline and is well known for its beaches, boating, sport
fishing, and seafood. Newport, famous for the summer "cottages" of
wealthy New Yorkers and for its annual music festivals, is the
state's best known tourist destination. Rhode Island offers hundreds
of acres of parkland, wildlife refuges, and hiking trails.
TO APPLY:
Send an email cover letter to [EMAIL PROTECTED] Eventually I
will ask for other refs, and a CV or transcript.
--
^_^_^_^_^_^_^_^_^_^_^_^_^_^_^_^_^_^_^_^_^_^_^_^_^_^_^
Anne Aaboe phone: (510) 643-7473
Undergraduate Advising Manager fax: (510) 643-2208
Molecular & Cell Biology email: [EMAIL PROTECTED]
2083 Valley Life Sciences, #3200 web: mcb.berkeley.edu/undergrad
Berkeley, CA 94720
----------------------------------------------------------------
The above message does not necessarily represent the views or opinions
of MCBcDNA, mcbUSA, the MCB Department, or UC Berkeley.
TO UNSUBSCRIBE FROM THIS LIST, send an email to [EMAIL PROTECTED] with
"unsubscribe mcb-infoline" in the message body. Please send questions
or comments to [EMAIL PROTECTED]
For a list of upcoming events and information about the student clubs:
MCBcDNA http://mcb.berkeley.edu/groups/mcbcdna/
mcbUSA http://mcb.berkeley.edu/groups/mcbusa/
