Hi
I need to clone a pfu amplified fragment in t-vector, in the vector manual it
is said that pfu pcr product must be purified before a tailing by tag because
pfu can remove a overhangs by its proofreading activity, but in commercial high
fidelity enzyme mixes which include a proofreading enzyme with tag we can use
pcr products directly for t/a ligation, so why in the latter case A overhangs
are not removed?
regards
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