Historians believe that in newspost
<mailman.46.1265484860.25217.meth...@net.bio.net> on Sat, 6 Feb 2010,
Azam Rahimpour <rahimpou...@yahoo.com> penned the following literary
masterpiece:
but in commercial high fidelity enzyme mixes which include a proofreading
enzyme with tag we can use pcr products directly for t/a ligation, so why
in the latter case A overhangs are not removed?
Because in a mix the ratio of Taq to proofreading enzyme will usually be
a minimum of 16:1 and possibly 64:1. At those ratios the error rate is
something like 3 fold better over Taq and the vast majority of products
have the A overhang. Basically too little proofreading enzyme to remove
all the overhangs
Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
Duncan Clark
GeneSys Ltd.
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