Hi Priscila, Phosphotyrosine may be quite easily detected; for a start, the PY20 antibody is a good choice. pS and pT are very tricky when you do not know the context (motif) or the identity of the modified protein, as pS and pT are not well recognized by antibodies against the phosphorylated amino acid, the signal depends a lot of the AA context. If you suspect some kinase (family) behind the effect, probing for the phosphorylation motif might be the best start. Beware that adjacent phosphorylable residues may mask each other in western blotting, dependent on their phosphorylation pattern. Cell Signaling has a good selection of anti-phospho-antibodies.
Good luck! Wo _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
