We routinely do phosphotyrosine blots (human proteins) with
anti-phosphotyrosine antibody clone 4G10 (many companies sell this). This is
the best and most cited antibody for phosphotyrosine and the most specific too.
It is of course not specific to any particular protein.
Jay
-----Original Message-----
From: methods-boun...@oat.bio.indiana.edu
[mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of Priscila Peña Diaz
Sent: Wednesday, March 03, 2010 7:19 AM
To: meth...@magpie.bio.indiana.edu
Subject: anti-phospho S, T & Y antibodies
Dear All:
I've been looking recently into phosphorylation of certain membrane proteins
after a peptide antibody (specific for my protein of interest) fails to
recognize the protein without prior treatment of the membrane (blotted
proteins) with phosphatase.
Most of the phosphorylation assays are usually performed with labelled
phosphate (32P) but there seem to be antibodies that can also do the job .
I was wondering if anybody has experience with these monoclonal anti-phospho S,
T & Y antibodies. They look pretty fancy (and expensive) but would like to hear
if anybody has had any actual experience with them.
kind regards
Priscila
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