I have used a 25-mer poly-dT oligo for a protein - single-stranded DNA titration that I studied by NMR. Now I am curious to find out if a poly-purine such as poly-dA or poly-dG will differ from the poly-dT poly-pyrimidine that I used first. But that's not my question: I notice that poly-dT appears to be a common choice for a single-stranded DNA oligo, and I wonder why? Maybe there's no reason, and personally I don't know why poly-dT should be "better" than any other homo-oligo for single stranded DNA. So I'm curious to know if anyone else knows if poly-dT, or any other poly-d(nucleotide) is any better than the others, as a single stranded DNA oligo?

Paul Phelan
Tufts University Department of Biochemistry
Boston

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