Probably you are talking about saporin (saponin is a detergent while saporin is a toxin). You cannot use Tween-20 or Triton X-100 as well, since they are also detergents and will lyse the cells. Best of luck Jay
-----Original Message----- From: methods-boun...@oat.bio.indiana.edu [mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of Joshua Silverstein Sent: Tuesday, July 27, 2010 11:27 AM To: meth...@magpie.bio.indiana.edu Subject: Saponin replacement? Hi Methodologists - I want to do a Live/Dead Assay on some HUVECs and the protocol calls for using saponin to kill control cells. I noticed it is a surfactant, can I use something like Tween-20 or Triton-X 100 instead that I have around the lab? Also what concentration would you use (I usually do 1% Triton to permeate the cells)? Thanks all, Josh _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
