For a live/dead assay you probably want the cells to remain as
adherent as possible. I've used 0.1% Triton X-100 for 10min, or 5%
DMSO for 12+ hrs.
On Jul 27, 2010, at 8:27 AM, Joshua Silverstein wrote:
Hi Methodologists -
I want to do a Live/Dead Assay on some HUVECs and the protocol
calls for
using saponin to kill control cells. I noticed it is a surfactant,
can I
use something like Tween-20 or Triton-X 100 instead that I have
around the
lab? Also what concentration would you use (I usually do 1% Triton to
permeate the cells)?
Thanks all,
Josh
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