I need to 5' phosphorylate some primes prior to PCR. Do I need to purify the primers after the kinase reaction or do you think I can just inactivate the enzyme at 65 degrees C followed by the PCR reaction? _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
- Polynucleotide 5' Kinase followed by PCR, purification needed? Jeff Hsu
