On Dec 2, 11:10 pm, Jeff Hsu <[email protected]> wrote: > I need to 5' phosphorylate some primes prior to PCR. Do I need to > purify the primers after the kinase reaction or do you think I can > just inactivate the enzyme at 65 degrees C followed by the PCR > reaction?
No separate inactivation needed, the initial melting step should kill PNK. Wo _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
