Hi Matt, You might look up some of the cloning vectors available on the registry of standard parts: I believe their standard vectors have a Biobrick cloning site is situated inside a lethal gene, so empty vectors lead to cell death upon establishment. http://partsregistry.org/Main_Page I believe they'll have a list of labs, one of which can ship you a sample pro bono. <http://partsregistry.org/Main_Page>All the best, Cathal
On 17 February 2011 15:06, mnr mnr <[email protected]> wrote: > Dear all > > I am looking for a small cloning vector (< 3.5 kbp) to clone my insert (> 8 > kbp) in E. coli . Does anyone have a reliable positive-selection vector > (i.e. selection based on inactivation of toxic genes and not blue-white > screening) that they can mail to me? Some that I found are part of kits > that > are a bit expensive for a small lab like mine. > > Thank you. > > Matt > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > -- letters.cunningprojects.com twitter.com/onetruecathal http://www.indiebiotech.com _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
