In addition to establishing the linear range of your film using a
standard curve as Wo suggests, be sure that your bands are not
saturated. Everything should be within the grayscale, no true black (for
black bands on white background) or white (for white bands on black
background).
Irit
On 2/18/2011 2:39 AM, WS wrote:
Don't know about photoshop. ImageJ (http://rsbweb.nih.gov/ij/) can do
the task very well. Despite it's free software, it is Java based and
runs in practically every computer environment. As a plus, the mailing
list and support is very active.
To convert the data that you will get into meaningful ones, you'll
probably need a standard curve within the actual picture. A problem
you might run into is the very low dynamic range of xray film and
thermal printouts. If you have the possibility, use fluorescence
scanners (e.g. Licor Odyssey) and cameras instead.
HTH
Wo
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