Dear all, This may have been asked before (maybe by me), but does anyone have any good advice on how to disrupt GST self-association? I often have trouble with preps. of GST-fusion proteins (when I need the intact fusion protein) being contaminated with GST that is very difficult to get rid of. I think this may be a result of "free" GST associating tightly with the GST-fusion. Has anyone else had this problem, and solved it?
Many thanks, Paul Phelan Tufts University Boston _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
