Hi DK, thanks! AARRGH! This could kill nicely any FPLC equipment (pumps, valves, columns, filters, even tubing) and sensitive assays where you possibly don't prepare half liter of 5M and clearly may see the turbidity. On the package, they just write "insoluble material <0.4%". A warning to use an appropriate filter before downstream processing would be more safe.
BTW, PBS is mainly for historic compatibility reasons: The solution once was used for a gradient; pH measurement then didn't show a huge deviation of pH due to GuHCl (slightly acidic). Wo _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
