Hi Sudheer, There are lots of kits available for concentrating protein. As long as your buffer is not denaturing or otherwise detrimental to your protein, then the concentration process itself should not modify the protein's structure.
Here are some options for you to consider. You should choose your concentration system based on the size of the protein (make sure your MWCO -- molecular weight cut-off -- is SMALLER than the size of your protein), the starting volume, and the target volume. http://www.millipore.com/lab_filtration/clf3/concentration&open&cid=Centrifugal-Amicon?gclid=CPW--tPB7qwCFWgaQgodiGRpiQ http://www.millipore.com/catalogue/module/c3259 Hope this helps, Irit On Dec 6, 2011, at 8:57 AM, sudheer sangeetham wrote: Hi Guys My recombinant protein is going to inclusion bodies so I am denaturing it Gn-HCl and renaturing with GSH and GSSG then finally purified the protein. After purification I am getting lower concentration of the protein of course it is in larger volume of the buffer. Now I wanted to concentrate the protein....Could anyone please tell me, the best and easy way to concentrate the protein with out modifying its original structure. Thank you in advance. -- Sudheer Babu.S Ph.D student Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list [email protected]<mailto:[email protected]> http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
