try using centricon with appropriate MW cut off or ethanol ppt or AS ppt can work for you to concentrate the protein.
On Tue, Dec 6, 2011 at 10:27 PM, sudheer sangeetham <[email protected] > wrote: > Hi Guys > My recombinant protein is going to inclusion bodies so I am denaturing it > Gn-HCl and renaturing with GSH and GSSG then finally purified the protein. > After purification I am getting lower concentration of the protein of > course it is in larger volume of the buffer. Now I wanted to concentrate > the protein....Could anyone please tell me, the best and easy way to > concentrate the protein with out modifying its original structure. > > Thank you in advance. > > -- > Sudheer Babu.S > Ph.D student > Institute of Biochemistry > Biological Research Center > Szeged,Hungary. > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > -- Sivakumar Uthandi PhD Associate Professor (Ag. Microbiology) Department of Agrl. Microbiology Tamil Nadu Agricultural University, Coimbatore 641 003, INDIA [email protected] 914226611294 (O), 914222442055 (R), 918903611294 (Mobile), 914226611394(Fx) _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
