indeed adding KCl to your phosphate buffer is highly recommended - and essential for keeping (most precious) proteins "alive" during freeze-thaw cycles (with NaCl only, phosphate buffer will precipitate and solution turns acidic - pH about 3 or so ...) cheers, David Minde 2012/1/3, Jayakumar, R <[email protected]>: > You might be referring to the use of PBS in animal systems like mice or rat. > When they mean PBS i.p. (was it written this way?), it means administration > of PBS into animal was via intraperitoneal injection or i.p (in the > abdmomen). Unless otherwise told, PBS contains both KCl and NaCl along > with Na2HPO4 and NaH2PO4. Refer the Sambrook manual or the current > protocols in Molecular biology or any standard tissue culture manual for the > PBS recipe. When the monobasic and dibasic salts are corrected added, the > pH will be around 7.5. If it is 6.4, your recipe is not right. > Jay > > > -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of sudheer sangeetham > Sent: Tuesday, January 03, 2012 11:53 AM > To: [email protected] > Subject: 10X PBS > > Hi People > > I have few doubts regarding phosphte buffer saline (PBS). Recently I found > one article related to my work, in that they used PBS ( Nacl/iP ) pH-7.4. > Nacl/iP does it mean, it should contain Na2HP04 and NaH2PO4 and Nacl but > not KCl, am I right? > > I have prepared the buffer like this > > NaH2PO4 ( 0.038M) > Na2HPO4.2H20 ( 0.162M) > NaCl (1.5 M ) but when i check the pH it was showing 6.4, is it correct? > did I do any mistake.... > > Could any one please tell me did i do any mistake? > > Thank you in advance > > -- > Sudheer Babu.S > Ph.D student > Institute of Biochemistry > Biological Research Center > Szeged,Hungary. > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > > > This email message may contain legally privileged and/or confidential > information. If you are not the intended recipient(s), or the employee or > agent responsible for the delivery of this message to the intended > recipient(s), you are hereby notified that any disclosure, copying, > distribution, or use of this email message is prohibited. If you have > received this message in error, please notify the sender immediately by > e-mail and delete this email message from your computer. Thank you. > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods >
-- David Minde, Drs Cellular Protein Chemistry Room 707 Department of Chemistry Faculty of Science Utrecht University Krytgebouw Padualaan 8 NL-3584 CH Utrecht The Netherlands office phone +31 30 253 4105 android +31 652614443 'Blood, toil, sweat and tears always were a good mixture'. (good friends among each other) _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
