Hi People I have few doubts regarding phosphte buffer saline (PBS). Recently I found one article related to my work, in that they used PBS ( Nacl/iP ) pH-7.4. Nacl/iP does it mean, it should contain Na2HP04 and NaH2PO4 and Nacl but not KCl, am I right?
I have prepared the buffer like this NaH2PO4 ( 0.038M) Na2HPO4.2H20 ( 0.162M) NaCl (1.5 M ) but when i check the pH it was showing 6.4, is it correct? did I do any mistake.... Could any one please tell me did i do any mistake? Thank you in advance -- Sudheer Babu.S Ph.D student Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
