In article <[email protected]>, [email protected] says... > > Dear all, > > I intend to use citrate buffer at 100 mM for some experiments, but > then I stumbled over a notice from Dionex, that citrate buffer can > modify proteins and peptides. > > However, when searching for references I was not able to find > information about it. Is this really a major artefact in citrate > buffer ? > > Does anyone have some information or references about it ?
I have never heard of that, but citrate complexes multivalent metal ions and allosterically regulates the activity of several enzymes. For both reasons it is used less often. Do you really need 100 mM of buffer? 10- 20 mM should suffice unless your reaction produces H+ or OH- as side- product. -- Car (noun): erratically moving obstacle on the road _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
