You can try a free sample of Advansta's WesternBright Quantum through this link:
http://advansta.com/WesternBright_Quantum_Sample_landing.html My Best, Sia Ghazvini 206.510.9636 ________________________________ From: Michael Sullivan <[email protected]> To: Cc: [email protected] Sent: Wednesday, January 18, 2012 5:07 PM Subject: Re: "Best" chemiluminiscent Westen detection? I've not done much comparison, but I've been using Bio-Rad's Immun-Star substrate for years. I use it with PVDF. There's an added enhancer for use with nitrocellulose, but I don't use NC, so I don't now how well that works. I've found the signal very long lived. Don't know about the price either. I don't do tons of westerns but I've been happy with the product, so cost has not been a big issue. We aren't set up for film anymore. We do detection with our Fotodyne gel doc system that also does luminescent detection. Mike On Jan 18, 2012, at 4:11 PM, DK wrote: > It's been over ten years since I last used ELC for Westerns. Back > then, we used Pierce's "ECL Plus" and a Kodak film of the type I can no > longer remember. > > It looks like I will be doing a bunch again in the upcoming months. > Ten years is a long time and I wonder if there were any new developments > or better/cheaper products available now. > > Super-duper sensitivity is not required. Strong preference is for the > substrate that has long-living signal (I was never abale to achieve it > with home-made luminol). Low price would be nice, too. AP or HRP > - does not matter. > > Oh, and what is a good inexpensive film to buy for ECL these days? > > Thanks! > > - Dima > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
