I've done quite a bit of ECL, including some comparisons among brands of reagents and film. Better results come at a higher price.
My go-to combination is Pierce SuperSignal Pico (more expensive), or Amersham/GE ECL Plus (less expensive), as far as reagent kits. For film, I like Molecular Technologies Classic Blue autorad film, distributed by MIDSCI. I've found that it has the best resolution at a reasonable price. Hope this helps, Irit On Jan 18, 2012, at 2:11 PM, DK wrote: > It's been over ten years since I last used ELC for Westerns. Back > then, we used Pierce's "ECL Plus" and a Kodak film of the type I can no > longer remember. > > It looks like I will be doing a bunch again in the upcoming months. > Ten years is a long time and I wonder if there were any new developments > or better/cheaper products available now. > > Super-duper sensitivity is not required. Strong preference is for the > substrate that has long-living signal (I was never abale to achieve it > with home-made luminol). Low price would be nice, too. AP or HRP > - does not matter. > > Oh, and what is a good inexpensive film to buy for ECL these days? > > Thanks! > > - Dima > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
