Dear experts, I would like to use TCEP (Tris-(2-carboxyethyl)-phosphine- hydrochloride, https://en.wikipedia.org/wiki/TCEP) for reducing disulfide bonds of a protein I want to couple to a maleimide containing spacer. The advantage of TCEP is that it doesn't react with maleimides, in contrast to BME or DTT, so the usual protocols do not require/provide for quenching of excess TCEP.
In the next step, I would like to couple this protein to another protein where leftovers of TCEP will harm the disulfide bonds. Is there any way to quench excess TCEP? Passing through a column of immobilized eg BSA comes into my mind. Any better ideas? Thanks! Wo _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
