Hello, I've been trying to gel purify my full length tRNA many different ways. With every attempt, I see degradation on my gel that I run to confirm purification.
Does anyone have any suggestions for methods that I should try which would not result in degradation of my purified tRNA? The samples are heated for 3mins at 70C prior to loading on the gel. This is our standard protocol for tRNA denaturation. Could this be causing my degradation? Any suggestions are welcome. Thank you, Rachel Schmidt _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
