Hi Rachel, What methods to purify and preserve your tRNA have you attempted so far? Best, Cathal
On 17/12/12 18:37, Rachel Schmidt wrote: > Hello, > > I've been trying to gel purify my full length tRNA many different ways. > With every attempt, I see degradation on my gel that I run to confirm > purification. > > Does anyone have any suggestions for methods that I should try which would > not result in degradation of my purified tRNA? > > The samples are heated for 3mins at 70C prior to loading on the gel. This > is our standard protocol for tRNA denaturation. Could this be causing my > degradation? > > Any suggestions are welcome. > Thank you, > Rachel Schmidt > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > -- www.indiebiotech.com twitter.com/onetruecathal PGP Public Key: 3B3DE808 (Verify before serious use) _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
