Hello everyone I wanted to check my protein concentration based on its extinction coefficient by using nanodrop rather than doing Bradford or Lowry methods. I was checking the article in one article, The protein concentrations were estimated using the extinction coefficient of 2.70 at 280 nm for a 1mg/ml solution, but to the same protein in another article they determined the concentration by giving 66350 /m/cm. If i want to check my protein concentration by using nanodrop which value I should concern and how did they get value of 2.7 ???
if anyone has idea please reply me Thanking you in advance Cheers -- Sudheer Babu.S Research Fellow Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
