Did anyone here used plasmid or cDNA clones from OriGene? is their product reliable?
Moreover, in many of their plasmid they denote the plasmid are not replicable by regular DNA purification method, is this possible? Looks to me they are just trying to get customers to buy the plasmid from them instead of purifying the plasmid indefinitely in their own bench I attached their 'explanation below' ;Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at [email protected] or by calling 301.340.3188 option 3 for pricing and delivery.; > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
