Isolation of RNA from formalin fixed paraffin tissue is doable. I have done it. Use High Pure RNA paraffin Kit from Roche.....
On Tue, Apr 16, 2013 at 12:00 AM, DK <[email protected]> wrote: > In article <[email protected]>, Pow Joshi < > [email protected]> wrote: > >Dear All, > > > >I have some cells fixed in 4% formalin (neutral I believe). I was > wondering > >if anyone has tried any RNA extraction on such cell/tissue samples, and if > >you have any wisdom including kits, tricks, solutions, problems that you'd > >share with me. I would appreciate it hugely. > > What's the downstream application? How old are fixed samples and > how were they stored? > > I have next to zero experience with what you are facing and I am > mostly curious as to what the real answer is but my guess is that > almost all of the RNA (say, 99%) is not recoverable. > > DK > > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
