On 16 April 2013 13:13, Shahrzad Jalali <[email protected]> wrote:
> Isolation of RNA from formalin fixed paraffin tissue is doable. I have done > it. Use High Pure RNA paraffin Kit from Roche..... > Would you be able to share some of the details and experiences. My cells are not in paraffin, so I can exclude the Xylene steps. However, I am concerned about the formaldehyde crosslinks...! Thanks, Pow > > > On Tue, Apr 16, 2013 at 12:00 AM, DK <[email protected]> wrote: > > > In article <[email protected]>, Pow > Joshi < > > [email protected]> wrote: > > >Dear All, > > > > > >I have some cells fixed in 4% formalin (neutral I believe). I was > > wondering > > >if anyone has tried any RNA extraction on such cell/tissue samples, and > if > > >you have any wisdom including kits, tricks, solutions, problems that > you'd > > >share with me. I would appreciate it hugely. > > > > What's the downstream application? How old are fixed samples and > > how were they stored? > > > > I have next to zero experience with what you are facing and I am > > mostly curious as to what the real answer is but my guess is that > > almost all of the RNA (say, 99%) is not recoverable. > > > > DK > > > > > > _______________________________________________ > > Methods mailing list > > [email protected] > > http://www.bio.net/biomail/listinfo/methods > > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
