Hello, I’m trying to run MISO on my RNA-seq libraries aligned with JAGuaR (Junction Alignments to Genome for RNA-Seq Reads). I keep running to following error: "Error: No GFF %s" %(gff_index_filename)”
I have installed all the modules. I’m also using the GFF files that is provided on MISO documentation page. This is a mouse data, aligned to mm10. Here is my full command: miso --run indexed/ test_chr.bam --output-dir testOutPut/ --read-len 75 --paired-end 300 20 I was wondering if you can help me in addressing this issue? Thank you, Ali
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