Dear Waldir, In terms of capturing internal anatomy, you need a volumetric imaging technique that's going to penetrate through the tissue. Depending on the size of the region you want to image, you can potentially user MRI, CT (both of which have high-resolution options, microMRI and microCT). I do not know anything about those fishes, but if genitalia are smallish (on the order 10-20mm length) and can be isolated, you might also look into optical imaging techniques such as optical projection tomography or 3D light-sheet microscopy, or even optical coherence tomography (OCT), all of which will require you clear your tissues, if you want to into anything more than a few millimeters deep. If you can sacrifice specimens, 3D histology can also be a destructive alternative
Extracting shape and structure information is a tricky question. Geometric morphometric analysis typically require a rigid coordinate framework. If your structures are plastic and easily malleable when handled, then you might have a hard time satisfying that assumption. However, people do conduct shape analysis on delicate organisms such as early or mid-gestation mouse embryos that doesn't have extensive mineralization. In that case, typically the whole thing is supported by some sort of matrix (e.g., agarose or hydrogel) to maintain their structural integrity. hth, M On Monday, March 30, 2020 at 3:30:29 PM UTC-7, waldirmbf wrote: > Dear morphometricians, > > Hope this email finds you all well and healthy during these delicate times. > > I am interested to research the shape and structure of female genitalia in > poecillid fishes. I wonder if anyone has any advice/literature on methods > how to extract shape and structure information from internal organs, such > as the female genitalia. Is anyone aware of any alternative methods other > CT scans? > > Any help is appreciated, > > Best Regards > > > > *Waldir M. Berbel-Filho, PhD* > > Postdoctoral Fellow > Oklahoma University, Norman, OK > > ResearchGate <https://www.researchgate.net/profile/Waldir_Berbel-Filho> | > Google > Scholar <https://scholar.google.com/citations?user=30a5mKUAAAAJ&hl=pt-BR> > | Publons <https://publons.com/researcher/1417914/waldir-m-berbel-filho/> > > -- > > > -- You received this message because you are subscribed to the Google Groups "Morphmet" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To view this discussion on the web visit https://groups.google.com/d/msgid/morphmet2/2b7f8cdf-ac43-4c7f-9df7-3cf8a88adc98%40googlegroups.com.
