Greetings!

I am interested in the measurement of local variation in the head shape of 
gilthead seabream populations, particularly on the aspect of statistical 
properties of parts within configurations. I found that Lory software, 
created by Márquez et al. (2012)*, provides the tools I am seeking for, 
however I have difficulties when trying to replicate the steps in the 
manual. I hope that someone that has the experience with it can share some 
light on the matter that are puzzeling me:

On the example data with *Drosophila* species authors have computed sample 
mean wing shape deformations for the two Drosophila species (Fig. 4).

o   How did they do that? Did they computed the group means in other 
programs or R packages and then imported them in Lory or there is a way to 
get them in Lory because I haven’t found an option to do that. One of the 
ways, as I see it, is to compute the mean shapes in geomorph and them 
import those means shapes in Lory to visualise those means shapes.

 After one iteration of the Delaunay algorithm, without any selection of 
evaluation  nodes, Lory found 74 nodes for evaluation. Based on that map of 
interspecific differences in local wing shape (Fig.5), on the extracted 
Jacobian determinants (74) ANOVA with Bonferroni correction for multiple 
comparison was performed which resulted with significant (stars) and 
non-significant (crosses) differences.

o   How did they computed that one shape with displayed interspecific 
differences?

o   Did they extracted Jacobians determinants from Lory and then performed 
ANOVA in some other program?

o   How did they know which node was significant or not? I suppose that you 
have to extract the coordinates that reflect their position on the map of 
interspecific differences or did I get it wrong?

 How did they computed the mean representation of intraspecific variance in 
Drosophila species? In Lory or in other program?

 Figures 7. and 8. represent interspecific correlations and interspecific 
differences and intraspecific variances. I suppose that these figures were 
computed in Coriandis software** on Jacobian determinants? I tried to 
import the data in Coriandis to explore if I could compute and get those 
figures but I am having problems with assigning group info and names to the 
imported non landmark data such as Jacobian determinants – no way I could 
manage to do that even when I tried to follow the footsteps in the manual 
that authors provided.

 

Thank you for your time and consideration.

 

Sincerely,

Igor

 

* Márquez, EJ., Cabeen, R., Woods, RP., Houle, D. 2012. The Measurement of 
Local Variation in Shape. Evol Biol, 39: 419 – 439.

** Márquez, EJ. & Knowles LL. 2007. Correlated evolution of multivariate 
traits: detecting codivergence across multiple dimensions. J. Evol. Biol., 
20: 2334 - 2348.

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