Dear Christina,
Judging from the output you provided, it seems like you are using MorphoJ. Am I
correct?
According to the classifiers, it says that the main effect for the among
individual variation requested in this field of the MorphoJ program is in fact
the classifier used for "Repeat". Therefore, it seems like the wrong classifier
was entered in the wrong field. In the design that you have, you have three
classifiers that correspond to three main effects in this nested two-way mixed
model ANOVA, "individual" that is the symmetric variation among individuals,
"Error 1" that is usually the error due to placing/replacing the specimen while
taking pictures or under another device for data acquisition ("imaging error")
and "Error 2" that is the error due to measuring the specimens ("digitizing
error"). Because you specified to MorphoJ that your data contain Object
Symmetry, it does not need an extra "side/reflection" main effect as it is
automatically taken into account by MorphoJ. Note that there is no analysis of
asymmetry in size for object symmetry because of constraints imposed by the
GPA, hence the output from MorphoJ for this analysis.
My guess is that you should enter your classifier "repeat" in "Error 2",
"image" in "Error 1" and a defined classifier for the variation among
individuals in "individuals".
I hope this helps,
Dr. Yoland Savriama
P/O Jernvall
Institute of Biotechnology
P.O. Box 56 (Viikinkaari 9)
FIN-00014 FINLAND
On Monday, August 11, 2014 1:00:03 PM UTC+3, Christina Berry wrote:
> Hey guys,
>
>
> I was wondering if someone would be able to help:
>
>
> I am attempting to do a Procrustes ANOVA on a pilot study I am doing on
> ticks, in order to see my imaging error vs. digitizing error so that I can
> either move on to the main study or tweak my current one. The output from
> the Procrustes ANOVA is:
>
>
>
> This analysis takes into account the object symmetry in the data.
>
>
> Classifiers used for the Procrustes ANOVA:
> Individuals: Repeat
> Error 1: Image
>
>
> Centroid size:
> Effect SS MS df F P
> (param.)
> Individual 5109.647411 1703.215804 3 0.05 0.9863
> Error 1 588604.834547 36787.802159 16
>
>
> Shape, Procrustes ANOVA:
> Effect SS MS df F P
> (param.)
> Individual 0.00406857 0.0000467652 87 2.94 <.0001
> Side 0.00449484 0.0001549945 29 9.76 <.0001
> Ind * Side 0.00138197 0.0000158847 87 0.13 1.0000
> Error 1 0.11081690 0.0001194148 928
>
>
> Shape, MANOVA tests of effects:
>
>
> Symmetric component of shape variation:
> Effect Pillai tr. P (param.)
> Note: the test for 'Individual' used the symmetric component of Error 1 as
> the 'error' effect.
>
>
> Asymmetry component of shape variation:
> Effect Pillai tr. P (param.)
>
>
> I can't seem to get a value for error 1 and error 2 isn't there, please help!
>
>
> All the Best
>
>
> Chrissy
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