Dear Alison

The residual (sums of squares, mean squares etc.) gives you the statistics for 
the second error effect.

I hope this helps.

Best wishes
Chris

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Christian Peter Klingenberg
Faculty of Life Sciences
University of Manchester
Michael Smith Building
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Web site: http://www.flywings.org.uk
E-mail: c...@manchester.ac.uk<mailto:c...@manchester.ac.uk>
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On 7 Oct 2016, at 19:36, Alison Foster 
<alison.foste...@gmail.com<mailto:alison.foste...@gmail.com>> wrote:


Hi everyone,

I'm trying to assess measurement error in a 2D file of 16 individual specimens 
with no object symmetry. For each specimen I have taken two different images 
and then copied each image twice, resulting in four images of each specimen. I 
have prepared ID strings as recommended on the MorphoJ online manual (for 
example phm1_1; phm1_2; phm2_1; phm2_2. The first three letters being the 
individual, the first digit being the image (Error 1) and the second digit the 
replica (Error 2)). Even though I've selected 'Ind', Error 1 and Error 2 in the 
Procrustes Anova text box, when I run it and get the results I don't have 
anything for Error 2. In the Reports tab, it says "The effect indicated for 
Error 2 has no degrees of freedom. The effect was dropped from the analysis"

I don't know why Error 2 isn't being calculated and would be very grateful for 
any suggestions or comments. I've appended the output from the Results below.

Many thanks,

Alison


Dataset: Humeri

Classifiers used for the Procrustes ANOVA:
Individuals: ind
Error 1: image

Centroid size:
Effect    SS    MS       df        F      P (param.)
Individual   67921.930157     4528.128677      15    22690.43      <.0001
Error 1          3.192978        0.199561      16       3.95      0.0005
Residual         1.618585        0.050581      32

Shape, Procrustes ANOVA:
Effect    SS  MS        df        F      P (param.)   Pillai tr.   P (param.)
Individual     0.05386010    0.0000897668      600     123.74      <.0001
Error 1        0.00046428    0.0000007254      640      13.93      <.0001
Residual       0.00006664    0.0000000521     1280





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