Hi, I did not receive anything. If you send some big files, please use a service like RapidShare. Also please write details about where is the problem.
Tomas On Dec 3, 2010, at 9:00 AM, Chunchao Zhang wrote: > Hi, Tomas > Did you get my email and files? > > chunchao > > > Quoting Chunchao Zhang <zhan...@umich.edu>: > >> Thank you Tom. My sample are pure histone H3 digested with trypsin. So >> spectrum won't be so complex as the whole cell extract. I don't think that >> there are so many chemical compounds with same formula. The raw data was >> generated from orbi-trap mass spec. Also, it looks the parameter doesn't >> change the results significantly. Typically, the mass detection was >> selected as exact mass with noise level: 3.00E4; FTML shoulder peak >> filterer: Resolution 30,000, gaussian; chrom construction was set as min >> time span: 7min, min height: 3.00E4, m/z tolerance: 0.005. >> >> chunchao >> >> >> >> Quoting Tomas Pluskal <plus...@oist.jp>: >> >>> Hi Chunchao, >>> >>> It is possible there are peaks with same m/z and different retention >>> times. There are many chemical compounds with same formula (= mass) but >>> different structure. >>> Please send us the XIC plots of the detected peaks (go to your peak list, >>> then right click on the peak, Show TIC). Or you can send your raw data >>> file and what parameters you used. >>> Without looking at the data, we cannot give you any advice. >>> >>> Tomas >>> >>> On Nov 30, 2010, at 1:49 AM, Chunchao Zhang wrote: >>> >>>> To whom it may concerns, >>>> I'm currently using mzmine software to exact ion chromatograph >>>> from LC-MS data. I first ran the Chromatogram builder algorithm to >>>> detect chromatograph then did deconvolution of chromatograms after >>>> peak detction. One of problem is I found multiple peaks with same m/z >>>> ratio in the peak list but with different RT, height and Area etc. >>>> Because my data came from high-resolution mass spec with high-mass >>>> accruracy, I would like to see one peak corresponding to one m/z ratio >>>> in the peak list. So I was wandering if there is anything wrong in >>>> what I did for XIC. Thanks! >>>> >>>> chunchao >>>> >>>> ------------------------------------------------------------------------------ >>>> Increase Visibility of Your 3D Game App & Earn a Chance To Win $500! >>>> Tap into the largest installed PC base & get more eyes on your game by >>>> optimizing for Intel(R) Graphics Technology. Get started today with the >>>> Intel(R) Software Partner Program. Five $500 cash prizes are up for grabs. >>>> http://p.sf.net/sfu/intelisp-dev2dev >>>> _______________________________________________ >>>> Mzmine-devel mailing list >>>> Mzmine-devel@lists.sourceforge.net >>>> https://lists.sourceforge.net/lists/listinfo/mzmine-devel >>>> >>> >>> =============================================== >>> Tomas Pluskal >>> G0 Cell Unit, Okinawa Institute of Science and Technology >>> 1919-1 Tancha, Onna-son, Okinawa 904-0412, Japan >>> TEL: +81-98-966-8684 >>> >>> >>> >>> >>> >>> >>> >> >> >> > =============================================== Tomas Pluskal G0 Cell Unit, Okinawa Institute of Science and Technology 1919-1 Tancha, Onna-son, Okinawa 904-0412, Japan TEL: +81-98-966-8684 ------------------------------------------------------------------------------ Increase Visibility of Your 3D Game App & Earn a Chance To Win $500! Tap into the largest installed PC base & get more eyes on your game by optimizing for Intel(R) Graphics Technology. Get started today with the Intel(R) Software Partner Program. Five $500 cash prizes are up for grabs. http://p.sf.net/sfu/intelisp-dev2dev _______________________________________________ Mzmine-devel mailing list Mzmine-devel@lists.sourceforge.net https://lists.sourceforge.net/lists/listinfo/mzmine-devel
