Hi,
I have created a combo box for the reference group.
Before you run the heat map module in MZmine you should install the package 
"gplots" in R.

Cheers,
Sandra

On 08/17/2011 11:34 AM, Tomas Pluskal wrote:
Hi Sandra,

When I did not set the reference group correctly, I got this exception:

java.lang.ArrayIndexOutOfBoundsException: 2
at 
net.sf.mzmine.modules.peaklistmethods.dataanalysis.heatmaps.HeatMapTask.groupingDataset(HeatMapTask.java:550)
at 
net.sf.mzmine.modules.peaklistmethods.dataanalysis.heatmaps.HeatMapTask.run(HeatMapTask.java:102)
at net.sf.mzmine.taskcontrol.impl.WorkerThread.run(WorkerThread.java:60)

When I set the reference group correctly, I got this one:

java.lang.IllegalStateException: The "gplots" R package couldn't be loaded - is 
it installed in R?
at 
net.sf.mzmine.modules.peaklistmethods.dataanalysis.heatmaps.HeatMapTask.run(HeatMapTask.java:137)
at net.sf.mzmine.taskcontrol.impl.WorkerThread.run(WorkerThread.java:60)

By the way, setting of the reference parameter would be better using a combo 
box, what do you think?

By the way #2, on any error your module just says "R error during the heat map 
creation". More detailed error message would be useful.

Cheers,

Tomas




On Aug 11, 2011, at 4:04 PM, Castillo Sandra wrote:

Hi Tomas,

I will solve this exception.
To run the heat map plot module first you have to define the sample groups in 
the sample parameter option (project->set sample parameters) (at least two 
groups) and define one of these groups as the reference group.
If the option "P-value legend" is selected, only the groups will be plot. If it 
is not selected, the samples will be plotted individually.

Sandra


On 08/11/2011 04:51 AM, Tomas Pluskal wrote:
Hi Sandra,

I tried to run it, but I got this exception:

[10:49:42|FINEST|MainMenu]: Starting module Heat map plot with parameters 
Output name: /tmp/aa.png, Output file type: png, Sample parameter: No 
parameters defined, Group of reference: asdas, Only identified rows: false, Use 
peak area: true, Scaling: true, Log: true, Show control samples: true, P-value 
legend: true, Size p-value legend: 5, Height: 10, Width: 10, Column margin: 10, 
Row margin: 10
Exception in thread "AWT-EventQueue-0" java.lang.NegativeArraySizeException
at 
net.sf.mzmine.modules.peaklistmethods.dataanalysis.heatmaps.HeatMapTask.groupingDataset(HeatMapTask.java:463)
at 
net.sf.mzmine.modules.peaklistmethods.dataanalysis.heatmaps.HeatMapTask.<init>(HeatMapTask.java:74)
at 
net.sf.mzmine.modules.peaklistmethods.dataanalysis.heatmaps.HeatMapModule.runModule(HeatMapModule.java:50)
at net.sf.mzmine.desktop.impl.MainMenu.actionPerformed(MainMenu.java:338)
at javax.swing.AbstractButton.fireActionPerformed(AbstractButton.java:2028)
at javax.swing.AbstractButton$Handler.actionPerformed(AbstractButton.java:2351)
at 
javax.swing.DefaultButtonModel.fireActionPerformed(DefaultButtonModel.java:387)
at javax.swing.DefaultButtonModel.setPressed(DefaultButtonModel.java:242)
at javax.swing.AbstractButton.doClick(AbstractButton.java:389)
at com.apple.laf.ScreenMenuItem.actionPerformed(ScreenMenuItem.java:95)
at java.awt.MenuItem.processActionEvent(MenuItem.java:627)
at java.awt.MenuItem.processEvent(MenuItem.java:586)
at java.awt.MenuComponent.dispatchEventImpl(MenuComponent.java:337)
at java.awt.MenuComponent.dispatchEvent(MenuComponent.java:325)
at java.awt.EventQueue.dispatchEventImpl(EventQueue.java:682)
at java.awt.EventQueue.access$000(EventQueue.java:85)
at java.awt.EventQueue$1.run(EventQueue.java:638)
at java.awt.EventQueue$1.run(EventQueue.java:636)
at java.security.AccessController.doPrivileged(Native Method)
at 
java.security.AccessControlContext$1.doIntersectionPrivilege(AccessControlContext.java:87)
at 
java.security.AccessControlContext$1.doIntersectionPrivilege(AccessControlContext.java:98)
at java.awt.EventQueue$2.run(EventQueue.java:652)
at java.awt.EventQueue$2.run(EventQueue.java:650)
at java.security.AccessController.doPrivileged(Native Method)
at 
java.security.AccessControlContext$1.doIntersectionPrivilege(AccessControlContext.java:87)
at java.awt.EventQueue.dispatchEvent(EventQueue.java:649)
at 
java.awt.EventDispatchThread.pumpOneEventForFilters(EventDispatchThread.java:296)
at 
java.awt.EventDispatchThread.pumpEventsForFilter(EventDispatchThread.java:211)
at 
java.awt.EventDispatchThread.pumpEventsForHierarchy(EventDispatchThread.java:201)
at java.awt.EventDispatchThread.pumpEvents(EventDispatchThread.java:196)
at java.awt.EventDispatchThread.pumpEvents(EventDispatchThread.java:188)
at java.awt.EventDispatchThread.run(EventDispatchThread.java:122)

Tomas

On Aug 10, 2011, at 11:02 PM, Castillo Sandra wrote:

Hi,
I just committed a new module to plot heat maps using R.
Any comment?

Sandra

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===============================================
Tomas Pluskal
G0 Cell Unit, Okinawa Institute of Science and Technology
1919-1 Tancha, Onna-son, Okinawa 904-0412, Japan
TEL:  +81-98-966-8684





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===============================================
Tomas Pluskal
G0 Cell Unit, Okinawa Institute of Science and Technology
1919-1 Tancha, Onna-son, Okinawa 904-0412, Japan
TEL:  +81-98-966-8684





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