Interesting ... take a look at:
Paphiopedilum cloning in vitro
Li-Chun Huang*, Chi-Jen Lin, Ching-I. Kuo,
Bau-Lian Huang, Toshio Murashige
Institute of Botany, Academia Sinica, Nankang, Taipei 115, Taiwan,
ROC
Accepted 5 December 2000
Abstract
A mericloning protocol that enables shoot multiplication and rooting of
Paphiopedilum orchids in
one step or in the same medium was developed by systematically assessing and
modifying the
nutrient supplements employed earlier for shoot proliferation by Huang [Am.
Orchid Soc. Bull. 57
(1988) 274]. Shoots of aseptically established seedlings obtained from a
commercial breeder were
used as explants. The new combination of supplements to the MS [Phyiol. Plant.
15 (1962) 473]
based medium consists of MT [Proc. First Intl. Citrus Symp. 3 (1969) 1155]
vitamins, glycine and
inositol; 13 mM BA (N6-benzyladenine); 1.6 mM NAA (a-naphthaleneacetic acid);
0.15 mM
adenine sulfateÁ2H2O; 1.23 mM NaH2PO4ÁH2O; 0.18 M sucrose; and 15%
(v/v) coconut
milk from
very young fruits. The coconut milk can be substituted with 1 g lÀ1 casein
hydrolysate or 10 g lÀ1
potato tuber sections. The investigation also disclosed that TDZ (N-phenyl-NH
-1,2,3-thiadiazol-5-yl
urea) inhibited shoot proliferation and rooting, and maltose depressed rooting.
The protocol enables
doubling the number of Paphiopedilum plants every 12 weeks. # 2001 Elsevier
Science B.V. All
rights reserved.
Keywords: Mericloning; Micropropagation; Orchid
The full text should be available through Elsevier or other sources.
Regards/Roger, In Bangkok
Quoting [EMAIL PROTECTED]:
> Hi Iris,
> I reckon "tissue culture" stand for flasking here. Otherwise you are right,
> true TC for paphs seem to be far away, mostly because of the difficulties
> extracting a meristem from a plant.
> Regards,
> Jean
>
> ...
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