Thanks Oliver, I may not be able to find the paper you referenced, but nevertheless I see your point. I agree with you about needing benchspace in order to bloom out the result of such breeding. Thansk for taking the time.
K Barrett N Calif, USA > > >Now... Is it too insane for me to ask for a dumbed down version as to how > >one does this? It seems awfully complicated. One would have to isolate >the > >gene/chromosome etc. Or does one just surmise its isolation from the > >outcome of the cross? > >With some difficulty: you might care to look at the Brassia napus paper >that i >referenced in the post. In essence, you need to cause a gamete (a pollen >cell) >to duplicate its genetic material, which you can do with colchicine and an >electric shock, amongst other stimuli. The resulting 'fake diploid' has to >be >nurded (a typo which I am going to let stand as appropriate - I mean to >type >'nursed') to adulthood through the usual mericlone process. Of course, you >have to do this in huge parallel to get the desired combination of genes, >and >unless you have a whole mol biol lab (and know which genes you want) you >have >to grow them all to adulthood to get the one that you want. >______________________________ > >Oliver Sparrow _________________________________________________________________ Mortgage refinance is hot 1) Rates near 30-yr lows 2) Good credit get intro-rate 4.625%* https://www2.nextag.com/goto.jsp?product=100000035&url=%2fst.jsp&tm=y&search=mortgage_text_links_88_h2a5f&s=4056&p=5117&disc=y&vers=743 _______________________________________________ the OrchidGuide Digest (OGD) [email protected] http://orchidguide.com/mailman/listinfo/orchids_orchidguide.com
