Hi Leon,
Depth of field is not something you can get very easily. If you have subjects with real depth the answer is to use Helicon Focus or ComZ to stack images. Photomicrography with a compound microscope is more difficult than with the lower magnification stereo type. I have stacked 50 images taken at 0.5 um vertical steps. If you're seriously interested in photo micrography email me off list and give me the details of what you want to do and the equipment you have.
I had hoped to hear from anyone in the group who has an *ist D attached to a compound microscope. I'd like to know if the shutter vibration is a serious drawback. I know the mirror can be fired in advance to help reduce this problem. But with a selection of other Pentax SLRs I found the shutter to be a real nuisance. I now use (for film) a dedicated Wild/Leica MPS 51 camera and MPS 48 electronic controller with a shutter that does not vibrate at all. But I'd like to move over to digital for routine work. I have all the attachments to fit Pentax bayonet bodies to my instruments.
D
Leon Altoff wrote:
On Thu, 28 Apr 2005 09:25:06 +0300, Don Williams wrote:
Hi all,
I've been off the list for some time. I need an digital camera that will be convenient to use on a microscope. My question is has anyone on the list used an *ist D for this?
Don,
I am currently looking for a microscope to attach to the *istD. The major problem with this is depth of field which is next to nothing at these magnifications. I am looking for a trinocular head with an aperture diaphram. I have connected the D to a microscope via a microscope adaptor and it worked ok, but I need more depth of field.
Leon
http://www.bluering.org.au http://www.bluering.org.au/leon
